Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate.

Liquid-liquid phase transitions in complex mixtures of proteins and other molecules produce crowded compartments supporting in vitro transcription and translation. We developed a method based on picoliter water-in-oil droplets to induce coacervation in Escherichia coli cell lysate and follow gene expression under crowded and noncrowded conditions. Coacervation creates an artificial cell-like en...

In vitro gene expression within membrane-free coacervate protocells.

Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix of a polysaccharide/polypeptide coacervate.

Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test

Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas ofIran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactionswere the main complaint after use of this vaccine. In a previous study, we could improve the vaccine byalum-precipitation Pasteurella multocida cells and removing the liquid phase. In this ...

Membrane-mimetic nanocarriers formed by a dipalmitoylated cell-penetrating peptide.

‏‎suppression of coke formation in thermal cracking by coke inhibitors‎‏

‏‎the main purpose of this research was to:1.develop a coking model for thermal cracking of naphtha.2.study coke inhibition methods using different coke inhibitors.developing a coking model in naphtha cracking reactors requires a suitable model of the thermal cracking reactor based on a reliable kinetic model.to obtain reliable results all these models shall be solved simultaneously.for this pu...